Journal: Diagnostics

Article Title: Alpha-Enolase (ENO1) Correlates with Invasiveness of Cutaneous Melanoma—An In Vitro and a Clinical Study

doi: 10.3390/diagnostics12020254

Figure Lengend Snippet: The expression level of ENO1 in the cell lysates from primary melanocytes and melanoma cell lines. Representative Western blots showing ENO1 and Akt 1/2/3 expression (for normalization) in protein lysates obtained from the primary human melanocytes (HEM) and indicated melanoma cell lines ( a ). Densitometric ENO1/Akt ratios are shown as mean values ( n = 3 except for HEM, n = 2) ± standard error of the mean (SEM) ( b ). The significance level was set at p = 0.001–0.0001 (***).

Article Snippet: Human epidermal melanocytes, adult (HEMa, 104−05A) and primary human epidermal melanocytes (lightly pigmented) (HEMn-LP, C0025C) were purchased from Cell Applications Inc (San Diego, CA, USA), and Cascade Biologics/Gibco (Carlsbad, CA, USA), respectively.

Techniques: Expressing, Western Blot

Journal: Journal of proteome research

Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures

doi: 10.1021/pr100164x

Figure Lengend Snippet: A. Cartoon depicting stages of melanoma progression in skin. Melanocytes reside in the epidermal/dermal junction. Early-stage radial growth phase melanoma cells descend into the papillary dermis and spread laterally. Invasion deep into the dermis characterizes melanomas in the vertical groth phase. B. Cartoon of a skin organ culture (SOC). SOCs are maintained at the air-liquid interface. The inset depicts a hematoxylin & eosin stained section collected on day 12 of culture.

Article Snippet: Normal human epidermal melanocytes (FC-0019, Lifeline Cell Technology, Walkersville, MD) were maintained in DermaLife Basal Medium with LifeFactors (LM-0027, Lifeline Cell Technology).

Techniques: Organ Culture, Staining

Journal: Journal of proteome research

Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures

doi: 10.1021/pr100164x

Figure Lengend Snippet: A. Quantitative RT-PCR analysis of the expression of tenascin-C (TN-C) mRNA in WM983-A (VGP) and WM1158 (MGP) melanoma cells compared to human melanocytes, propagated in vitro. The data are expressed as the mean±standard deviation from three independent experiments. B. Immunoblot analysis depicting the level of TN-C in the VGP and MGP melanoma cell lines and melanocytes. C. Immunofluorescence analysis of melanocytes and VGP and MGP melanoma cell lines, probed with antibody to TN-C (pseudocolored green) and actin (pseudocolored red). The cells were counterstained with fluorescent DAPI (pseudocolored blue).

Article Snippet: Normal human epidermal melanocytes (FC-0019, Lifeline Cell Technology, Walkersville, MD) were maintained in DermaLife Basal Medium with LifeFactors (LM-0027, Lifeline Cell Technology).

Techniques: Quantitative RT-PCR, Expressing, In Vitro, Standard Deviation, Western Blot, Immunofluorescence

Journal: Journal of proteome research

Article Title: Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures

doi: 10.1021/pr100164x

Figure Lengend Snippet: A. Quantitative RT-PCR analysis of α-actinin-4 (ACN4) expression in the WM983-A (VGP) and WM1158 (MGP) melanoma cell lines and in melanocytes. The data are expressed as the mean ±standard deviation from three independent experiments. B. Immunoblot analysis of ACN4 expression in the VGP and MGP melanoma cell lines and in melanocytes. C. Immunofluorescence analysis of melanocytes and VGP and MGP melanoma cell lines, probed with antibody to ACN4 (pseudocolored green) and actin (pseudocolored red). The cells were counterstained with fluorescent DAPI (pseudocolored blue).

Article Snippet: Normal human epidermal melanocytes (FC-0019, Lifeline Cell Technology, Walkersville, MD) were maintained in DermaLife Basal Medium with LifeFactors (LM-0027, Lifeline Cell Technology).

Techniques: Quantitative RT-PCR, Expressing, Standard Deviation, Western Blot, Immunofluorescence